CAS号：

9039-53-6

中文名称：

尿激酶

英文名称：

Urokinase (peptidolytic)

中文别名：

尿激酶;
ucon(R) htf 14;
Amioca WCS;
C*Pharm 12018;
Cato 225;
Cato 240;
Cato 270;
Cerestar SF 04201;
Farinex WM 85;
Film Kote 54;
Honen Alpha Waxy Starch;
Kosol;
Pectin, amylo;
Starch, waxy;
UNII-4XO4QFV777;
Ultraamylopectin N;
Ultrasperse A;
WCS;
Waxy 7350;
Waxy Alpha Y;
Waxy corn starch;
Waxy maize starch;
Waxy starch;
Corn starch amylopectin;
alpha-D-glucopyranosyl-(1->4)-[alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->6)]-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranose;
Amylopectin;
Insulinum dalanatum [INN-Latin];
Insulyl;
Intesulin B;
Inyectable neutro de insulina;
Inyectable neutro de insulina [INN-Spanish];
Iszilin;
Musulin;
Solute neutre injectable d'insuline;
Solute neutre injectable d'insuline [INN-French];
Insulin;
Triolein hydrolase;
Tween esterase;
Tween hydrolase;
Tweenase;
UNII-8MYC33932O;
UNII-FQ3DRG0N5K;
Lipase of Rhizopus arrhizus var. Delemar;
Plant lipase;
Animal lipase;
Lipozyme TL IM;
Lipozyme 435;
Lipozyme CALB L;
Lipozyme RM IM;
Palatase 20000L;
Recombinant lipase A;
Triacylglycerollipaza;
Lipase

英文别名：

Urokinase (peptidolytic);
Urokinase (peptidolytic) (9CI);
Kinase (enzyme-activating), uro- (9CI);
2-Chain urokinase;
Abbokinase;
Actosolv;
Breokinase;
Cytokinase;
Double-chain urokinase-type plasminogen activator;
E.C. 3.4.21.31;
E.C. 3.4.21.73;
E.C. 3.4.99.26;
Persolv;
Plasminokinase, urinary;
Pro-HGF convertase;
Pro-hepatocyte growth factor convertase;
Purochin;
Two-chain urokinase;
Two-chain urokinase-type plasminogen activator;
Ukidan;
Urokinase;
Urokinase medac;
Urokinase plasminogen activator;
Urokinase-like plasminogen activator;
Urokinase-type plasminogen activator;
Uronase;
Win 22005;
Win-Kinase

贝尔斯坦号/MDL：

MFCD00132566

EINECS：

232-917-9

合成制备方法

1.将新鲜男性尿液（pH=6.5以下，细菌数1000个/ml以下）冷却至10℃以下，用3mol/L的氢氧化钠调节pH=8.5，静置1h，虹吸出上清液，用3mol/L的盐酸再中和至pH=5～5.5。然后每升酸化尿液中加入硅藻土10g，在5℃以下搅拌吸附。吸附物用5℃的冷水洗涤后装柱，用0.02%的氨水洗涤至流出液变清，再用含有氯化钠（1mol/L）的氨水（0.02%）洗脱尿激酶，在洗脱液浑浊时开始收集，收集量为每吨尿约15L洗脱液。洗脱液用饱和磷酸二氢钠调节pH值为8，用氯化钠调节电导为22mS。然后通过QAESephadex色谱柱，收集流出液，色谱柱再用磷酸缓冲液洗涤，并与流出液合并。流出液用乙酸调节pH值为4.2，再用蒸馏水调电导为16～17mS。然后通过CMC色谱柱，用pH=4.2的乙酸乙酸钠缓冲溶液洗涤色谱柱，再用含氯化钠（0.1mol/L）的氨水（0.1%）洗脱尿激酶。将洗脱液在4℃对水透析除盐24h，透析液离心去沉淀，冻干，即可得尿激酶。

2.组织培养法 （美）