---

Section I.Chemical Product and Company Identification
    Chemical Name        Crystal Violet    
    [Ion association reagent for spectrophotometric analysis.]
    Portland OR
    Basic Violet 3
    Synonym
    Chemical Formula        C25H30ClN3•9H2O    
    CAS Number        548-62-9    

---

Section II.        Composition and Information on Ingredients    
    Chemical Name        CAS Number Percent (%)        TLV/PEL        Toxicology Data    
    Crystal Violet        548-62-9        ----------        This chemical is classified as a        Rat LD50 (oral) 420mg/kg    
    [Ion association reagent for spectrophotometric analysis.]
    carcinogen. There is no        Mouse LD50 (oral) 96mg/kg    
    acceptable exposure limit for a        Rabbit LD50 (intraperitoneal)    
    carcinogen.        5mg/kg    

---

Section III. Hazards Identification
    Acute Health Effects        Toxic if ingested or inhaled. Avoid prolonged contact with this material. Overexposure may result in serious illness or death.    
    Irritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the
    eye is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or,
    occasionally, blistering.
    Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound.
    CARCINOGENIC EFFECTS :
    Chronic Health Effects
    Rat-TDLo - ROUTE: Oral; DOSE: 10950 mg/kg/2Y continuous
    TOXIC EFFECTS:
    Tumorigenic - Carcinogenic by RTECS criteria
    Endocrine - Thyroid tumors
    Mouse-TDLo - ROUTE: Oral; DOSE: 25750 mg/kg/2Ycontinuous
    TOXIC EFFECTS:
    Tumorigenic - Carcinogenic by RTECS criteria
    Kidney, Ureter, and Bladder - Tumors
    Tumorigenic Effects - Ovarian tumors
    MUTAGENIC EFFECTS : Not available.
    TERATOGENIC EFFECTS :
    DEVELOPMENTAL TOXICITY:
    Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
    TOXIC EFFECTS:
    Specific Developmental Abnormalities -Musculoskeletal system
    Specific Developmental Abnormalities -Urogenital system
    Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
    TOXIC EFFECTS:
    Effects on Embryo or Fetus - Fetotoxicity (except death, e.g., stunted fetus)
    Effects on Embryo or Fetus - Fetal death
    Specific Developmental Abnormalities -
    Musculoskeletal system
    Repeated exposure to an highly toxic material may produce general deterioration of health by an accumulation in one or
    many human organs.

---

Section IV.        First Aid Measures    
    Eye Contact        Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15    
    minutes. Get medical attention.
    Skin Contact        In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing    
    and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
    Inhalation        If the victim is not breathing, perform mouth-to-mouth resuscitation. Loosen tight clothing such as a collar, tie, belt or    
    waistband. If breathing is difficult, oxygen can be administered. Seek medical attention if respiration problems do not
    improve.
    Continued on Next Page
    Crystal Violet
    [Ion association reagent for spectrophotometric analysis.]
    Ingestion        INDUCE VOMITING by sticking finger in throat. Lower the head so that the vomit will not reenter the mouth and throat.    
    Loosen tight clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-to-mouth
    resuscitation. Examine the lips and mouth to ascertain whether the tissues are damaged, a possible indication that the toxic
    material was ingested; the absence of such signs, however, is not conclusive.

---

Section V.        Fire and Explosion Data    
    Not available.
    May be combustible at high temperature.        Auto-Ignition    
    Flammability
    Flammable Limits        Not available.    
    Flash Points        Not available.    
    Combustion Products        These products are toxic carbon oxides (CO, CO2), nitrogen oxides (NO, NO2), halogenated compounds.    
    WARNING: Highly toxic HCl gas is produced during combustion.
    Fire Hazards        Not available.    
    Risks of explosion of the product in presence of mechanical impact: Not available.
    Explosion Hazards
    Risks of explosion of the product in presence of static discharge: Not available.
    Fire Fighting Media        SMALL FIRE: Use DRY chemical powder.    
    and Instructions        LARGE FIRE: Use water spray, fog or foam. DO NOT use water jet.    
    Consult with local fire authorities before attempting large scale fire-fighting operations.

---

Section VI.        Accidental Release Measures    
    Spill Cleanup        Environmentally hazardous material. Toxic material. Irritating material. Carcinogenic material. Light-sensitive material.    
    Stop leak if without risk. DO NOT get water inside container. DO NOT touch spilled material. Use water spray to reduce
    Instructions
    vapors. Prevent entry into sewers, basements or confined areas; dike if needed. Eliminate all sources of ignition. Consult
    federal, state, and/or local authorities for assistance on disposal.

---

Section VII. Handling and Storage
    ENVIRONMENTAL HAZARD. TOXIC. IRRITANT. CARCINOGEN. LIGHT-SENSITIVE. Keep locked up. Keep away from
    Handling and Storage
    heat. Mechanical exhaust required. When not in use, tightly seal the container and store in a dry, cool place. Avoid
    Information
    excessive heat and light. DO NOT ingest. Do not breathe dust. Wear suitable protective clothing. If ingested, seek medical
    advice immediately and show the container or the label. Treat symptomatically and supportively.
    Always store away from incompatible compounds such as oxidizing agents.

---

Section VIII. Exposure Controls/Personal Protection
    Engineering Controls        Use process enclosures, local exhaust ventilation, or other engineering controls to keep airborne levels below recommended    
    exposure limits. If user operations generate dust, fume or mist, use ventilation to keep exposure to airborne contaminants
    below the exposure limit.
    Personal Protection        Splash goggles. Lab coat. Dust respirator. Gloves. A MSHA/NIOSH approved respirator must be used to avoid inhalation of    
    the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
    This chemical is classified as a carcinogen. There is no acceptable exposure limit for a carcinogen.
    Exposure Limits

---

Section IX. Physical and Chemical Properties
    Physical state @ 20°C        Green crystal.        Solubility        Not available.    
    Not available.
    Specific Gravity
    Molecular Weight        407.98 (Anh)        Partition Coefficient        Not available.    
    Boiling Point        Not available.        Not available.    
    Vapor Pressure
    Melting Point        Not available.        Vapor Density        Not available.    
    Not available.        Not available.    
    Refractive Index        Volatility    
    Critical Temperature        Not available.        Odor        Not available.    
    Not available.        Not available.    
    Viscosity        Taste    

---

Section X.        Stability and Reactivity Data    

---

This material is stable if stored under proper conditions. (See Section VII for instructions)
    Stability
    Conditions of Instability        Avoid excessive heat and light.    
    Incompatibilities        Reactive with strong oxidizing agents, strong reducing agents.    
    Continued on Next Page
    Crystal Violet
    [Ion association reagent for spectrophotometric analysis.]

---

Section XI.        Toxicological Information    
    BO9000000
    RTECS Number
    Eye contact. Ingestion. Inhalation. Skin contact.
    Routes of Exposure
    Toxicity Data        Rat LD50 (oral) 420mg/kg    
    Mouse LD50 (oral) 96mg/kg
    Rabbit LD50 (intraperitoneal) 5mg/kg
    Chronic Toxic Effects        CARCINOGENIC EFFECTS :    
    Rat-TDLo - ROUTE: Oral; DOSE: 10950 mg/kg/2Y continuous
    TOXIC EFFECTS:
    Tumorigenic - Carcinogenic by RTECS criteria
    Endocrine - Thyroid tumors
    Mouse-TDLo - ROUTE: Oral; DOSE: 25750 mg/kg/2Ycontinuous
    TOXIC EFFECTS:
    Tumorigenic - Carcinogenic by RTECS criteria
    Kidney, Ureter, and Bladder - Tumors
    Tumorigenic Effects - Ovarian tumors
    MUTAGENIC EFFECTS : Not available.
    TERATOGENIC EFFECTS :
    DEVELOPMENTAL TOXICITY:
    Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
    TOXIC EFFECTS:
    Specific Developmental Abnormalities -Musculoskeletal system
    Specific Developmental Abnormalities -Urogenital system
    Rat-TDLo - ROUTE: Oral; DOSE: 100 mg/kg; DURATION: female 6-15D of pregnancy
    TOXIC EFFECTS:
    Effects on Embryo or Fetus - Fetotoxicity (except death, e.g., stunted fetus)
    Effects on Embryo or Fetus - Fetal death
    Specific Developmental Abnormalities -
    Musculoskeletal system
    Repeated exposure to an highly toxic material may produce general deterioration of health by an accumulation in one or many
    human organs.
    Acute Toxic Effects        Toxic if ingested or inhaled. Avoid prolonged contact with this material. Overexposure may result in serious illness or death.    
    Irritating to eyes and skin on contact. Inhalation causes irritation of the lungs and respiratory system. Inflammation of the eye
    is characterized by redness, watering, and itching. Skin inflammation is characterized by itching, scaling, reddening, or,
    occasionally, blistering.
    Follow safe industrial hygiene practices and always wear proper protective equipment when handling this compound.

---

Section XII.        Ecological Information    
    Ecotoxicity        Not available.    
    Hexamethyl-p-rosaniline chloride's production and uses as a dye and topical antibacterial medication, both human and
    Environmental Fate
    veterinary, may result in its release to the environment through various waste streams. If released to air, an estimated vapor
    pressure of 1.9X10-14 mm Hg at 25 deg C indicates hexamethyl-p-rosaniline chloride will exist solely in the particulate phase
    in the ambient atmosphere. Particulate-phase hexamethyl-p-rosaniline chloride may be removed from the atmosphere by wet
    and dry deposition. Hexamethyl-p-rosaniline chloride has a UV max of 590 nm indicating a potential for direct photolysis in the
    environment. If released to soil, hexamethyl-p-rosaniline chloride is expected to have no mobility based upon an estimated
    Koc of 4X10+6. Hexamethyl-p-rosaniline chloride is a cationic salt and cations generally adsorb to organic carbon and clay
    more strongly than their neutral counterparts. Volatilization from moist soil surfaces is not expected to be an important fate
    process based upon an estimated Henry's Law constant of 3.1X10-16 atm-cu m/mole. If released into water,
    hexamethyl-p-rosaniline chloride isexpected to adsorb to suspended solids and sediment based upon the estimated Koc.
    Measurable transformation rates of 0.002 to 0.012/hour, that correspond to a half-life range of 2.4 to 14.4 days, were
    observed in 42 batch culture die-away tests using natural water samples from four sites. The photoreaction of
    hexamethyl-p-rosaniline chloride in water is reported to give p-dimethylamino phenol, 4,4'-bis dimethylamino benzophenone,
    the leuco- and demethylated derivative of hexamethyl-p-rosaniline chloride. Volatilization from water surfaces is not expected
    to be an important fate process based upon this compound's estimated Henry's Law constant. An estimated BCF of 3.2
    suggests the potential for bioconcentration in aquatic organisms is low. Occupational exposure to hexamethyl-p-rosaniline
    chloride may occur through inhalation of dust and dermal contact with this compound at workplaces where
    hexamethyl-p-rosaniline chloride is produced or used. General population exposure may occur via use of topical medications
    and consumer products containing this compound. (SRC) [Peer Reviewed]

---

Section XIII. Disposal Considerations
    Recycle to process, if possible. Consult your local or regional authorities. You may be able to dissolve or mix material with a
    Waste Disposal
    combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber system. Observe all
    federal, state, and local regulations when disposing of the substance.

---

Section XIV. Transport Information
    DOT Classification        CLASS 9: Miscellaneous hazardous material.    
    UN: 3077
    PIN Number
    Proper Shipping Name        Environmentally hazardous substance, solid, n.o.s.    
    Packing Group (PG)        III    
    DOT Pictograms
    Continued on Next Page
    Crystal Violet
    [Ion association reagent for spectrophotometric analysis.]

---

Section XV. Other Regulatory Information and Pictograms
    TSCA Chemical Inventory        This compound is ON the EPA Toxic Substances Control Act (TSCA) inventory list.    
    (EPA)
    WHMIS Classification        On DSL.    
    (Canada)
    EINECS Number (EEC) 208-953-6
    EEC Risk Statements        R23/24/25- Toxic by inhalation, in contact with skin and if swallowed.    
    R36/37/38- Irritating to eyes, respiratory system and skin.
    R45- May cause cancer.

---

SECTION 16 - ADDITIONAL INFORMATION
N/A